Molecular identification key for pest species of Scirtothrips (Thysanoptera: Thripidae).
نویسندگان
چکیده
Effective plant quarantine and biological control initiatives require rapid and accurate identification of exotic and potentially invasive taxa that may cause high economic losses or environmental damage. The genus Scirtothrips Shull includes several species that are serious agricultural pests, and, because of their minute size and cryptic behavior, prone to undetected transport through international trade of plant material. Although assigning specimens to the genus Scirtothrips is straightforward using traditional taxonomic methods, identification of species is much more difficult and requires expert knowledge of the genus. Furthermore, the validity of some Scirtothrips species is questionable. Therefore, an easy, accurate, and highly reliable technique is desirable for Scirtothrips identification. Here, we provide a simple molecular key based on the internal transcribed spacer regions 1 and 2 (ITS1 and ITS2) of nuclear ribosomal DNA. Individual specimens can be identified by amplification of their ITS1 and ITS2 regions with general primers and determining the size of the products by using standard agarose gel electrophoresis, followed in some instances by DNA digestion with the restriction enzymes SacII or PspOM I. The advantage of this identification system is that nonspecialists are able to quickly and cheaply identify individual specimens. Material analyzed for this work was collected in the United States (California), India, South Africa, Kenya, Mexico, Guatemala, Honduras, Nicaragua, Costa Rica, Panama, Australia, New Zealand and Raiatea (Society Islands French Polynesia). We have identified seven pest species with the molecular-based methods described here. It is hoped that this system can be extended to other members of the genus as their ITS1 and ITS2 sequences become available. We also provide molecular confirmation for two new Scirtothrips species, one species from Honduras and one species from New Zealand.
منابع مشابه
Relationships Among Species of Scirtothrips (Thysanoptera: Thripidae, Thripinae) Using Molecular and Morphological Data
Results of analyses of molecular sequence (COI, 28S-D2) and morphology (21 character states) data, both alone and combined, were used to determine relationships between 18 species of Scirtothrips (Thysanoptera: Thripidae, Thripinae). Two species of Neohydatothrips from Panama were used as the outgroup. Five major pest Scirtothrips were included in these analyses: S. aurantii, S. citri, S. dorsa...
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The Scirtothrips genus-group is here considered to comprise 11 genera, and an identification key to these is presented. These genera are Ajothrips Bhatti, Anascirtothrips Bhatti, Biltothrips Bhatti, Cercyothrips Morgan, Drepanothrips Uzel, Ephedrothrips zur Strassen, Kenyattathrips Mound, Parascirtothrips Masumoto & Okajima, Scirtidothrips Hood, Scirtothrips Shull and Siamothrips Okajima. One g...
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ورودعنوان ژورنال:
- Journal of economic entomology
دوره 99 5 شماره
صفحات -
تاریخ انتشار 2006